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. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: Science. 2016 Jan 28;351(6276):933–939. doi: 10.1126/science.aad0314

Fig. 1. lL-17a increase in mothers subjected to MIA leads to elevated IL-17Ra mRNA expression in the offspring.

Fig. 1

(A) Serum concentrations of IL-6 (n = 3–5 mice per group, 2 independent experiments) at 3 or 24 hours after PBS or poly(l:C) injection into pregnant dams at E12.5. (B) Serum concentrations of maternal IL-17a (n = 4–8 mice per group, 2 independent experiments) at E14.5 in PBS- or poly(l:C)-injected mothers, pretreated with or without IL-17a blocking antibodies. (C and D) Relative IL-6 (C) and IL-17a (D) mRNA expression in cells isolated from placenta/decidua of PBS- or poly(l:C)-treated mothers at E14.5 and cultured in vitro for 24 hours,. The results are representative of three independent experiments. For each probe set, relative mRNA expression of one biological replicate from PBS-treated dams was set at 1. Real-time PCR analysis of relative expression of indicated genes compared to the level of Gapdh in cells from PBS-treated dams. (E) Supernatant concentrations of IL-17a from ex vivo cultured mononuclear cells, isolated from placenta/decidua of PBS- or poly(l:C)-treated pregnant dams. Stim refers to PMA and lonomycin stimulation. (F and G) Relative IL-17Ra (F) and IL-17Rc (G) mRNA levels in E14.5 male fetal brain, derived from PBS- or Poly(l:C)-injected mothers, pretreated with isotype control (Cont) or IL-17a blocking antibodies (anti-IL-17a). The relative mRNA fold change, compared to the PBS and Cont-treated group, is plotted on the y-axis (n = 7 (PBS, Cont), n = 7 (PBS, anti-IL-17a), n = 7 (Poly(l:C), Cont), n = 7 (Poly(l:C), anti-IL-17a); from 2–3 independent experiments). (H) In situ hybridization with an IL-17Ra RNA probe in E14.5 male fetal brains derived from PBS- or poly(l:C)- injected mothers. Images are representative of four independent experiments. (I) Relative signal intensity for images shown in (H). Scale bar represents 100 µm. (A, B, E, F and G) One-way ANOVA with Tukey post-hoc tests. (C, D and I) Student's t test. **P < 0.01. Graphs show mean ± SEM.