Fig 4. Microfluidic-based cell invasion assay.
(A) Illustration of the microfluidic device. (B) Photo of the microfluidic device filled with red stain. (C) Experimental design. Fibroblasts (red) were cultured in the cell culture channel of a microfluidic device.(D) Cell invasion assay using a microfluidic device. The invasion areas of CAF-A1 and CAF-A2 were significantly greater than that of NF. ** P< 0.01, *** P< 0.001, n = 5. Scale bar = 100 μm
