Fig 1. In vitro activities of anti-CXCR4 monoclonal antibody LY2624587.

A. Inhibition of [¹²⁵I] SDF-1α binding to CXCR4 by LY2624587 (square) or control IgG (triangle). Ligand binding assay was developed with CCRF-CEM cells, and the CCPM in the Y axis stands for corrected counts per minute. B. Dose dependent inhibition of SDF-1 induced GTP binding by LY2624587 (solid circle) or control IgG (triangle). GTP binding assay was developed with CCRF-CEM membrane. C. Inhibition of SDF-1 induced cell migration (chemotaxis) in U937 cells by LY2624587. D. Inhibition of SDF-1 induced cell migration (chemotaxis) in CCRF-CEM cells by LY2624587. E and F. LY2624587 has no apparent agonist activity. LY2624587 was tested in GTPγS35 binding assay (E) or cell migration assay (F) in agonist mode. The GTPγS35 binding assay was developed with CCRF-CEM cell membrane, and the SDF-1-induced cell migration assay was developed with U937 cells as described under “Materials and Methods.”