| Bacterial Strains |
|
|
| Escherichia coli |
|
|
| One Shot® TOP10 |
F- mcrA Δ(mrr-hsdRMS-mcrBC)φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG |
Invitrogen |
|
P. syringae pv. syringae
|
|
|
| B728a |
Wild-type, bean pathogen; Rifr
|
[1] |
| B728aΔsalA
|
salA mutant derivative of B728a, Rifr
|
[34, 35] |
| B728aΔsyrF
|
syrF mutant derivative of B728a, Rifr
|
This study |
| B728aΔsyrG
|
syrG mutant derivative of B728a, Rifr
|
This study |
| B728aΔsyrFΔsyrG
|
syrF and syrG mutant derivative of B728a, Rifr
|
This study |
| B728aΔgacS
|
gacS mutant derivative of B728a, Rifr
|
[36] |
| Plasmids |
|
|
| pE2602 |
pENTR/D-TOPO 6.80 kb region carrying syrG, Kmr
|
This study |
| pE2607 |
pENTR/D-TOPO 6.62-kb region carrying syrF, Kmr
|
This study |
| pKD13 |
Template plasmid containing FRT-flanked nptII
|
[37] |
| pLVCD |
Gateway destination vector for mating with P. syringae; pBR322 derivative with mob genes from RSF1010; Tcr Apr Cmr
|
[38] |
| pLV2602 |
pLVCD carrying syrG; Tcr Apr
|
This study |
| pLV2607 |
pLVCD carrying syrF; Tcr Apr
|
This study |
| pLV2602-FP |
pLVCD carrying upstream and downstream regions of syrG fused to nptII; Tcr Apr Kmr
|
This study |
| pLV2607-FP |
pLVCD carrying upstream and downstream regions of syrF fused to nptII; Tcr Apr Kmr
|
This study |
| pPROBE-KT’ |
Promoter-probe vector with pVS1/p15a replicon and gfp reporter, Kmr
|
[39] |
| pPKT::syrG752 |
pPROBE-KT’ carrying syrG along with 752-bp upstream; Kmr
|
This study |
| pPKT::syrG552 |
pPROBE-KT’ carrying syrG along with 552-bp upstream; Kmr
|
This study |
| pPKT::syrG452 |
pPROBE-KT’ carrying syrG along with 452-bp upstream; Kmr
|
This study |
| pPKT::syrG352 |
pPROBE-KT’ carrying syrG along with 352-bp upstream; Kmr
|
This study |
| pPKT::syrG352-10 |
pPROBE-KT’ carrying syrG along with 352-bp upstream with the potential -10 region replaced with CTGCAG; Kmr
|
This study |
| pPKT::syrG352-35 |
pPROBE-KT’ carrying syrG along with 352-bpupstream with the potential -35 region replaced with CTGCAG; Kmr
|
This study |
| pPKT::syrG252 |
pPROBE-KT’ carrying syrG along with 252-bp upstream; Kmr
|
This study |
| pPKT::syrG202 |
pPROBE-KT’ carrying syrG along with 202-bp upstream; Kmr
|
This study |
| pPKT::syrG102 |
pPROBE-KT’ carrying syrG along with 102-bp upstream; Kmr
|
This study |
| pPKT::syrG52 |
pPROBE-KT’ carrying syrG along with 52-bp upstream, Kmr
|
This study |
| pPKT::syrF
|
pPROBE-KT’ carrying syrF along with 1.3-kb upstream; Kmr
|
This study |
| pPKT::syrF1000 |
pPROBE-KT’ carrying syrF along with 1.0-kb upstream; Kmr
|
This study |
| pPKT::syrF800 |
pPROBE-KT’ carrying syrF along with 800-bp upstream; Kmr
|
This study |
| pPKT::syrF600 |
pPROBE-KT’ carrying syrF along with 600-bp upstream; Kmr
|
This study |
| pPKT::syrF600-10 |
pPROBE-KT’ carrying syrF along with 600-bp upstream with the potential -10 region replaced with CTGCAG; Kmr
|
This study |
| pPKT::syrF600-35 |
pPROBE-KT’ carrying syrF along with 600-bp upstream with the potential -35 region replaced with CTGCAG; Kmr
|
This study |
| pPKT::syrF500 |
pPROBE-KT’ carrying syrF along with 500-bp upstream; Kmr
|
This study |
| pPKT::syrF252 |
pPROBE-KT’ carrying syrF along with 252-bp upstream; Kmr
|
This study |
| pPKT::syrF202 |
pPROBE-KT’ carrying syrF along with 202-bp upstream; Kmr
|
This study |
| pPKT::syrF152 |
pPROBE-KT’ carrying syrF along with 152-bp upstream; Km |
This study |
| pPKT::syrF102 |
pPROBE-KT’ carrying syrF along with 102-bp upstream; Kmr
|
This study |
| pPKT::syrF52 |
pPROBE-KT’ carrying syrF along with 52-bp upstream; Kmr
|
This study |
| pMEKm12 |
E. coli and P. syringae pv. syringae overexpression vector, Kmr
|
[40] |
| pMK::syrG
|
pMEKm12 carrying the syrG gene in-frame fused to malE; Kmr
|
This study |
| pMK::syrG583 |
pMEKm12 carrying 583-bp of the syrG N-terminal region fused to malE; Kmr
|
This study |
| pMK::syrF
|
pMEKm12 carrying the syrF gene in-frame fused to malE; Kmr
|
This study |
| pMK::syrF583 |
pMEKm12 carrying 583-bp of the syrF N-terminal region fused to malE; Kmr
|
This study |
| pRK2073 |
Helper plasmid; Spr Trmr
|
[41] |
