Table 2. Effect of salA, syrG, and syrF mutants on gfp reporter gene activity for salA, syrG, syrF, and syrB1.a.
| Strain (reporter fusion) | Gene | GFP fluorescence ± SE |
|---|---|---|
| B728a (salA::gfp) | salA | 43,044 ± 203 |
| B728aΔsalA (salA::gfp) | salA | 10,055 ± 357 |
| B728aΔsyrG (salA::gfp) | salA | 42,991 ± 425 |
| B728aΔsyrF (salA::gfp) | salA | 38,106 ± 454 |
| B728a (syrG::gfp) | syrG | 45,776 ± 773 |
| B728aΔsalA (syrG::gfp) | syrG | 4,175 ± 237 |
| B728aΔsyrG (syrG::gfp) | syrG | 6,121 ± 449 |
| B728aΔsyrF (syrG::gfp) | syrG | 47,875 ± 389 |
| B728a (syrF::gfp) | syrF | 44,857 ± 718 |
| B728aΔsalA (syrF::gfp) | syrF | 2,202 ± 223 |
| B728aΔsyrG (syrF::gfp) | syrF | 6,163 ± 239 |
| B728aΔsyrF (syrF::gfp) | syrF | 7,713 ± 281 |
| B728a (syrB1::gfp) | syrB1 | 47,266 ± 2,158 |
| B728aΔsalA (syrB1::gfp) | syrB1 | 9,653 ± 626 |
| B728aΔsyrG (syrB1::gfp) | syrB1 | 24,347 ± 522 |
| B728aΔsyrF (syrB1::gfp) | syrB1 | 29,458 ± 345 |
a The pPROBE-KT’ vector was used to construct pPKT fusions of specific genes to the gfp reporter. GFP fluorescence were averaged over three technical replicates of three biological samples followed by the standard error of the averaged values.