Skip to main content
. 2016 Mar 8;12(3):e1005927. doi: 10.1371/journal.pgen.1005927

Fig 5. HDR1 interacts with OsK4.

Fig 5

(A) HDR1 directly interacted with kinase OsK4 in the Y2H system. (B) Exon1 and Exon2 of HDR1, but not Exon3, directly interacted with OsK4 in yeast cells. (C) BiFC analyses of the interactions of HDR1 with OsK4 in Arabidopsis protoplast. YFP signals resulted from the physical association of HDR1 with OsK4 proteins, not negative pairs, in the nuclei. Scale bar = 10μm. (D) Co-immunoprecipitation of HDR1 with OsK4 in rice plant. Total protein extracted from the leaves expressing FLAG-HDR1 and WT, and then FLAG-HDR1 was immunoprecipitated with anti-FLAG, followed by western blotting to analyze the OsK4 with OsK4 antibody.