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. 2016 Mar 8;11(3):e0150336. doi: 10.1371/journal.pone.0150336

Fig 3. ASPH upregulated the expression of cyclin D1 expression and accelerated cell proliferation.

Fig 3

(A) Relative CCND1 mRNA expression in H1, RBE, SSP25, and ETK1 cells infected with lentivirus containing shLuc or shASPH. (B) Immunoblotting of ASPH, cyclin D1, and intracellular domain of Notch1 (ICN) in RBE-shLuc, RBE-shASPH, ETK1-shLuc, and ETK1-shASPH transfected cells with or without overexpression of ICN. (C) Relative cell proliferation in RBE-shLuc, RBE-shASPH, ETK1-shLuc, and ETK1-shASPH in the presence or absence of 10 μM of the γ-secretase inhibitor DAPT using the MTT assay. (D) Relative cell proliferation rate in RBE-shLuc, RBE-shASPH, RBE-shASPH transfected cells overexpressing ICN. ***, p <0.001; **, p <0.01; *, p <0.05.