Fig 1. Filament formation by mutants of the LCD of Nab3.

(A) A portion of the wildtype (Nab3¹³⁴) and glutamine to glutamate substituted (Nab3^134QE) LCD of S. cerevisiae Nab3 is shown. The extent of the deletion of Q₁₆ run and the terminal hnRNP-C-like domain (Δα) are also shown. The region of Nab3 with structural homology to human hnRNP-C is underlined. (B) Transmission electron microscopy images of filaments from the various purified versions of Nab3¹³⁴ are shown. (C) Purified Nab3¹³⁴ and Nab3^134QE were run on a 12% SDS-PAGE gel (left panel) next to molecular weight markers and stained with Imperial^TM stain (note this polypeptide binds Coomassie blue-based dyes poorly). Thioflavin T-binding and fluorescence (right panel) was measured for Nab3¹³⁴ and Nab3^134QE as described in Materials and Methods.