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. 2016 Mar 8;11(3):e0150865. doi: 10.1371/journal.pone.0150865

Fig 3. Nab3 whose LCD lacks 24 glutamines cannot support cell viability.

Fig 3

(A) Yeast bearing a URA3-marked plasmid containing NAB3 and either a LEU2-marked plasmid encoding wildtype NAB3 (DY357) or one encoding nab3 with the twenty-four glutamine to glutamate substitutions shown in Fig 1 (nab3Q>E) (DY382), were grown in SC leu- and plated onto media containing FOA or synthetic complete (SC) media. (B) An anti-Nab3 western blot is shown on lysates from yeast strains DY357, DY368, DY384, and DY382 (lanes 1–4, respectively) containing the indicated versions of Nab3. (Control strains harboring as their only copy of NAB3 either an 11 or 14 glutamine to glutamate substitutions are shown in lanes 2 and 3).