Fig 4. The high-scoring DR candidates for glioblastoma among the FDA-approved drugs that were predicted based only on the expression signatures.

(A) DR scores, (B) the fraction of significantly inhibited cells summarizing the results of (C), (C) the anti-proliferative activities (% growth inhibition) for the four glioblastoma cell lines (four cell lines of TG98, A172, U251MG, and U87MG) and the eight patient-derived primary cells (the GBLs) at 10 μM, (D) in silico prediction scores for BBB transport based on http://www.cbligand.org/BBB. The red asterisk indicates experimental support for passing the BBB according to the literature. Overall, anti-proliferative activities across glioblastoma cells strongly correlated with the rankings by the DR score. Most DR candidates were shown to be able to pass the BBB.