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. 2016 Mar 8;11(3):e0151065. doi: 10.1371/journal.pone.0151065

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© 2016 Tyagi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Co-localization images of PvTRAg36.6 with apicoplast, rhoptry and micronemal markers in P.vivax natural infections. (A) Fluorescence pattern observed after co-immuno staining of P.vivax parasite with anti-PvTRAg36.6 (green) and anti-PfClpP (red) recognizing apicoplast in schizont (A, upper panel) as well as in free merozites (A, lower panel). (B) Co-immunostaining of anti-PvTRAg36.6 (green) with anti-PvRII (red) recognizing microneme in a schizont, and (C) Co-immunostaining of anti-PvTRAg36.6 (green) with anti-PvAARP (red) recognizing rhoptry neck in a schizont. The parasite nuclei were stained with DAPI (blue). Overlay shows the images merged with bright field.