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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2015 Jul 10;89:1–7. doi: 10.1016/j.freeradbiomed.2015.07.004

Figure 4. Endothelial Nox4 prevents MIG expression.

Figure 4

Aorta were harvested and mRNA of the T regulatory marker FoxP3 (A) and the T effector marker T-bet (B) were measured (mean ± SE; n = 4 – 6; *p < 0.05). Plasma was collected from mice fed with a Western diet for 24 weeks and cytokine levels were measured by ELISA in ApoE−/− and ApoE−/− + EC Nox4 mice (C) (mean ± SE; n = 8 –10; *p < 0.05). Aorta were harvested and MIG mRNA was measured (D). Mouse lung endothelial cells (MLECs) were harvested from both EC Nox4 transgenic mice (Nox4 TG) and littermate controls (WT). MLECs were treated for 24h with mouse IFNγ (12.5 ng/mL) and MIG mRNA expression was measured (normalized to HPRT; mean ± SE; n = 3; *p < 0.05) (E). Human aortic endothelial cells (HAECs) were either transduced by control virus or Nox4 expressing virus. After transduction cells were treated for 24h with human IFNγ (12.5 ng/mL) and harvested for gene expression of MIG (normalized to HPRT; mean ± SE; n = 3; **p < 0.01) (F).