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. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: ALTEX. 2014 Jul 14;31(4):441–477. doi: 10.14573/altex1406111

Fig. 8. Reconstructing human skin.

Fig. 8

Normal fibroblasts and keratinocytes are isolated from, e.g., human foreskin and expanded in monolayer culture. Fibroblasts are embedded into a collagen matrix (step 1) and the collagen is allowed to contract (growth medium). Keratinocytes are seeded on top and medium is changed to differentiation medium (step 2). Finally the culture is lifted to the air-medium interface (step 3). Total culture duration is about 14 days. For the reconstruction of human epidermis step 1 is omitted and keratinocytes are seeded on a supporting membrane.