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. Author manuscript; available in PMC: 2016 Mar 8.
Published in final edited form as: Immunity. 2013 Mar 14;38(4):705–716. doi: 10.1016/j.immuni.2013.02.013

Figure 2. SARM1 Deficiency Limits LACV-Mediated Neuronal Death but Not Virus Infection or Replication in Neurons.

Figure 2

(A) Neurons were treated with Sarm1 siRNA or nontargeting (NT) siRNA starting at 3 days postculture. Twenty-four hours later, cells were infected with LACV and cell viability was measured by MTT assay at 36 hpi. SARM1 protein expression in neurons treated with NT or Sarm1 siRNA is shown in inset on right.

(B) Neurons from WT and Sarm1−/− mice were infected with LACV, and cell viability was measured at 24, 30, and 36 hr by MTT assay.

(C–E) Cells were stained for DNA fragmentation by TUNEL analysis at 36 hpi and counted as percent positive as described in Figure 1. (D and E) Immunofluorescence analysis of neurons from (D) WT and (E) Sarm1−/− mice infected with LACV at 36 hpi with TUNEL-stained nuclei (green) and LACV (red). The relative lack of cells in (D) compared to (E) is due to cell death. Scale bar represents 25 μm.

(F) Viral RNA expression in neurons at 36 hpi as determined by real-time PCR. All data are shown as mean ± SEM of 3–6 samples per group per time point. Data are representative of 2 or 3 replicate experiments per panel. See also Figure S3.