Figure 5.

Evaluation of the treatment effects on luciferase activity in a peritoneal dissemination xenografted mouse model bearing MKN45/Luc cells. We monitored the bioluminescence intensity of the peritoneal dissemination and compared the total fluorescence in single or repeated integrated therapy groups with that of a control group. Before treatment, mice with approximately the same luciferase intensity were selected and randomized into 7 groups (at least 4 animals per group). A, the schedule of the integrated therapy regimen is shown. B, the time course of the bioluminescence images of tumor-bearing mice after treatment in vivo. C, D, the quantitative luciferase activity in mice after treatment. The total fluorescence of the integrated therapy group was remarkably suppressed compared with that of controls. The luciferase activity of the mice treated with repeated integrated therapy remained significantly lower than in other groups (n= 4 or 5 mice in each treatment group; *P< 0.05 vs control group using one-way repeated ANOVA).