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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Dev Biol. 2016 Feb 10;411(2):172–182. doi: 10.1016/j.ydbio.2016.02.006

Figure 5.

Figure 5

Most of the sperm in the ampulla underwent acrosomal exocytosis. A) Representative image of an ampulla after ovulation. B) The eggs were easily observed through the oviductal wall. C-E) Representative images of a fertilized egg where it was possible to observe the fluorescence of the tail. F-H) Representative images of two sperm in the vicinity of an egg. Both sperm lost their acrosomes. I-K) Representative images of two sperm in the vicinity of an egg. In this case, one sperm was acrosome intact (AI) while the other already underwent acrosomal exocytosis (AR). L-N) Representative example of one acrosome reacted sperm swimming through 3 ovulated eggs (indicated by arrows). BF: bright field image,