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. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: J Neurovirol. 2015 Sep 25;22(2):179–190. doi: 10.1007/s13365-015-0383-6

Fig 3 — Treatment of SH-SY5Y cells with Nef exosomes from HEK293 cells induces production of Nef and increases expression of Amyloid. a SH-SY5Y cells were treated with Nef exosomes (red line, red axis) or the control preparation (red dashed line) for the times indicated and cells were harvested and tested by RT-PCR for the presence of nef mRNA. Cells treated with Nef exosomes (blue bars, blue axis) or mock control (blue hatched bars) were also tested for the presence of APP mRNA. b SH-SY5Y cells treated with Nef exosomes were also tested by ELISA for the presence of Nef protein (red line, red axis) and Aβ_1-40 (blue bars). The dashed line and hatched bars were treated with exosomes from mock-transfected cells. The results shown are the average of three independent experiments. c SH-SY5Y cells were treated with 80μg/ml of cyclo-heximide at the same time as the addition of Nef exosomes or were untreated and tested for the presence of Nef-GFP at 0, 8, 16 and 24h. The table below the gel shows the results of densitometry from an average of three independent experiments.

Fig 3 — Treatment of SH-SY5Y cells with Nef exosomes from HEK293 cells induces production of Nef and increases expression of Amyloid. a SH-SY5Y cells were treated with Nef exosomes (red line, red axis) or the control preparation (red dashed line) for the times indicated and cells were harvested and tested by RT-PCR for the presence of nef mRNA. Cells treated with Nef exosomes (blue bars, blue axis) or mock control (blue hatched bars) were also tested for the presence of APP mRNA. b SH-SY5Y cells treated with Nef exosomes were also tested by ELISA for the presence of Nef protein (red line, red axis) and Aβ_1-40 (blue bars). The dashed line and hatched bars were treated with exosomes from mock-transfected cells. The results shown are the average of three independent experiments. c SH-SY5Y cells were treated with 80μg/ml of cyclo-heximide at the same time as the addition of Nef exosomes or were untreated and tested for the presence of Nef-GFP at 0, 8, 16 and 24h. The table below the gel shows the results of densitometry from an average of three independent experiments.