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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Analyst. 2016 Mar 21;141(6):1939–1946. doi: 10.1039/c5an02468d

Fig. 4.

Fig. 4

Effect of varying amylin concentrations on the peak area percentages of the A42003-amylin complex peak. (A) Representative electropherograms of amylin assay. The concentration of A42003 was fixed at 250 nM while the concentration of murine amylin increased from 0 to 50 nM as indicated in the figure. (B) The variation of the complex peak area percentage obtained as a function of amylin concentration. (C) Calibration curve of complex peak area percentages vs. amylin concentrations from 0 to 10 nM. The data followed a linear response, Y = 0.1191 X, r2 = 0.9990. (D) A zoomed-in view of the complex peak produced by incubation with 0.5 nM amylin, highlighted (A). All samples were incubated at 37 °C for 16 hours before injection. Each point represents the average of 3 runs with error bars corresponding to ± 1 SD.