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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Mol Cancer Ther. 2016 Feb 1;15(3):460–470. doi: 10.1158/1535-7163.MCT-15-0456

Figure 2.

Figure 2

Inhibition of T-type Ca2+ channels induces apoptosis and lowers survivin expression in ovarian cancer cells. (A) The sub-G1 population in cells treated with 10 μmol/L Mib for 24 hours was determined by flow cytometry. (B) Western blot analysis of markers of apoptotic cell death in total cell extracts from ovarian cancer cells treated with 10 μmol/L Mib for 24 hours. (C) Expression of BIRC5 mRNA determined by quantitative RT-PCR in ovarian cancer cells treated with Mib for 24 hours at 6 μmol/L (A2780) or 10 μmol/L (A2780Cis and IGROV-1). (D) Expression of BIRC5 mRNA determined by quantitative RT-PCR in A2780 and A2780Cis cells 72 hours after transfection with siRNA against T-type channels isoforms. All values are the mean ± SEM from ≥ two independent experiments; **, P < 0.01; ***, P < 0.001.