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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Allergy Clin Immunol. 2015 Oct 21;137(3):889–898.e6. doi: 10.1016/j.jaci.2015.08.040

FIG 5.

FIG 5

A MyD88/PYK2/LYN complex mediates TLR9 induced p85 PI3K recruitment to CD19. A, The phosphorylation of CD19 was analyzed in lysates of purified peripheral blood B cells from a healthy control stimulated with CpG with or without addition of a src family kinase inhibitor (PP2), a PYK2 inhibitor (AG-17) or a BTK inhibitor (PCI32765). Lysates of healthy control CD20+ tonsil B cells (B) or EBV-transformed B-cell lines (C) stimulated or not with CpG for 30 minutes were immunprecipitated with anti-MyD88 (B), anti-PYK2 (B) or an isotype control antibody (IgG) followed by immunoblot analysis. D, EBV-transformed B-cells from a healthy control were stimulated or not for 30 min with CpG without inhibitor or in the presence of a src family kinase inhibitor (PP2) or a PYK2 inhibitor (AG-17). Lysates were immunoprecipitated with anti-CD19 or an isotype control antibody (IgG) followed by immunoblotting against p85 PI3K.