Figure 1.

Effects of LG2055 feeding on the regulation of lifespan, motility, and expression of aging markers in Caenorhabditis elegans. (a) Effects of LG2055, Lactobacillus gasseri JCM1131^T (LG1131^T) or Lactobacillus helveticus JCM1120^T (LH1120^T) on the regulation of lifespan of the worms were determined. Control worms were fed with Escherichia coli OP50. Data are obtained by three independent experiments with 100 worms grown at 20 °C per group. Lifespan survival statistics are in Table S1. Pharynx pumping (b) or body bending (c) of the worms was counted after feeding with LG2055, LG1131^T, or LH1120^T for 10 days after feeding stopped. Accumulation of lipid droplets was detected by oil red O staining (d), and dye intensity was measured (e) in the worms fed with LG2055 or OP50 for 5 or 10 days. Accumulation of lipofuscin was detected by autofluorescence (f), and fluorescence intensity was measured (g) in the worms fed with LG2055 or OP50 for 5 or 10 days. Results are shown by the SEM of three independent experiments with 10 worms in each group; statistically evaluated by an unpaired Student's t‐test (**P < 0.01, and ***P < 0.001).