Figure 4.

Effects of LG2055 feeding on the Caenorhabditis elegans p38 MAPK pathway. (a–d) Lifespan analysis of tir‐1 (ok1052), nsy‐1 (ag3), sek‐1 (km3), and pmk‐1 (km25). Data are obtained by three independent experiments with 100 worms grown at 20 °C per group. Lifespan survival statistics are in Table S2. (e) Activation of PMK‐1 by LG2055 feeding. After the feeding of LG2055 or OP50 for 10 days, extracts prepared from each animal were immunoblotted (IB) with antiphospho p38. *P < 0.05, Student's t‐test.