Table 1.
Enrichment analysis of alternative spliced genes between 4‐ and 18‐month‐old animals across five tissues
| Category | P value/score | No. of genes | % of genes on lista |
|---|---|---|---|
| GO biological processesWG | P value | ||
| Macromolecule metabolic process | 1.00E‐04 | 77 | 48.7 |
| Cellular macromolecule metabolic process | 1.00E‐04 | 72 | 45.6 |
| RNA processing | 1.00E‐04 | 17 | 10.8 |
| mRNA processing | 1.00E‐04 | 13 | 8.2 |
| RNA splicing | 3.00E‐04 | 11 | 7.0 |
| KEGG pathwaysWG | P value | ||
| Spliceosome | 1.00E‐04 | 8 | 5.1 |
| Cell cycle | 3.96E‐02 | 5 | 3.2 |
| mRNA surveillance pathway | 3.96E‐02 | 4 | 2.5 |
| RNA transport | 4.78E‐02 | 5 | 3.2 |
| Protein processing in ER | 5.45E‐02 | 5 | 3.2 |
| Canonical pathwaysIPA | P value | ||
| EIF2 signaling | 1.44E‐02 | 5 | 3.2 |
| ATM signaling | 1.51E‐02 | 3 | 1.9 |
| GDP‐L‐fucose biosynthesis II (from L‐fucose) | 1.78E‐02 | 1 | 0.6 |
| Protein ubiquitination pathway | 2.15E‐02 | 6 | 3.8 |
| Estrogen receptor signaling | 2.44E‐02 | 4 | 2.5 |
| Molecular and cellular functionsIPA | P value | ||
| RNA post‐transcriptional modification | 1.55E‐04 – 4.78E‐02 | 11 | 7.0 |
| Cellular development | 2.29E‐04 – 4.91E‐02 | 14 | 8.9 |
| Protein synthesis | 9.87E‐04 – 3.52E‐02 | 15 | 9.5 |
| Cellular assembly and organization | 1.08E‐03 – 4.38E‐02 | 20 | 12.7 |
| Cell cycle | 1.74E‐03 – 4.38E‐02 | 13 | 8.2 |
| Diseases and disordersIPA | P value | ||
| Cancer | 1.55E‐09 – 4.78E‐02 | 124 | 78.5 |
| Gastrointestinal disease | 1.55E‐09 – 4.08E‐02 | 73 | 46.2 |
| Organismal injury and abnormalities | 1.61E‐07 – 4.38E‐02 | 75 | 47.5 |
| Reproductive system | 1.61E‐07 – 4.38E‐02 | 59 | 37.3 |
| Infectious disease | 1.74E‐03 – 4.38E‐02 | 13 | 8.2 |
| NetworksIPA and network‐associated functions | Score | ||
| Cellular assembly and organization, developmental disorder, hereditary disorder | 48 | 24 | 15.2 |
| RNA post‐transcriptional modification; hereditary disorder, ophthalmic disease | 46 | 23 | 14.6 |
| Organ morphology, reproductive system development and function, embryonic development | 38 | 20 | 12.7 |
| Cancer, gastrointestinal disease, inflammatory disease | 31 | 18 | 11.4 |
| Cell signaling, post‐translational modification, protein synthesis | 28 | 16 | 10.1 |
a
Number of genes on list = 158, WGEnrichment analysis performed with WebGestalt, – Subcategory, IPAEnrichment analysis performed with ingenuity pathway analysis.