Figure 2.

TRAIL treatment damages hair cells and SGNs in cultured murine cochlear explants. (A) Representative images of P4 explants from the same cochlear region that received either 0.1 m PBS (‘nontreated’, NT), 1 μg mL⁻¹ TRAIL, or 1 μg/mL TRAIL and 4 μg mL αDR5 Ab. MyoVIIa (green) marks hair cells. Tuj1 (red) marks spiral ganglion neuron (SGN) neurites and somata. Scale bar: 100 μm (top two rows) or 50 μm (bottom row). (B) The number of inner hair cells (IHC) per 100 μm of cochlear length. (C) The number of outer hair cells (OHC) per 100 μm of cochlear length. (D) The number of SGN neurites per 100 μm of cochlear length. (E) The number of SGNs per 10⁴ μm². (F) The distribution of the area of the SGN somata. *P < 0.05, **P < 0.05. n = number of different explants. A total of 12 mice were used in these experiments for Figure 2 and Figure S1. Data are plotted as mean ± SD (B–F).