Figure 1.

MicroRNAs differentially expressed in aged hippocampus of mouse. (A) Heat map of 80 miRNAs showing ≥ twofold change in the hippocampus between any two of the three age groups. Small RNA samples were pooled out of eight hippocampi from four individual mice for each age group. This sample was used for sequencing as a single library and subsequent bioinformatical analysis. (B) Scattered volume plot of miRNAs differentially expressed between the 2‐ and 18‐month‐old hippocampi with ≥ twofold change. The size of the marker represents the magnitude of expression according to the formula $\sqrt{(\mathrm{RPM}2\mathrm{m})\ast (\mathrm{RPM}18\mathrm{m})}$. The miRNAs marked in red were selected for qPCR validation. (C) qPCR validation of representative miRNAs differentially expressed between the 2‐ and 18‐month‐old hippocampi. miRNA levels were measured from three independent samples. Shown are the average and standard deviation for each miRNA, *P < 0.05. This result reflects the reliability of NGS data.