Figure 2.

Downregulation of Eph/ephrin‐mediated axon guidance pathway genes in aged hippocampus. (A) qPCR validation of representative mRNAs in the Eph/ephrin‐mediated axon guidance pathway. mRNA levels were measured from three independent samples. Shown are the average and standard deviation for each mRNA, *P < 0.05. (B) Diagram depicting signaling relationships among the Eph/ephrin pathway genes, including those showing differential expression in hippocampus between the 2‐ and 18‐month‐old mice (red, upregulated in aged hippocampus; green, downregulated in aged hippocampus). (C) The protein levels of EphB2, EphA4, RhoA, and tubulin (internal control) in the 2‐ and 18‐month‐old hippocampi as measured by immunoblotting with antibodies specific to each protein. EphB2 protein level was downregulated by 60% in the aged hippocampus compared with that in the young hippocampus. n = 3 (three different animals), *P < 0.05 **P < 0.005.