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. 2016 Jan 22;15(2):380–388. doi: 10.1111/acel.12444

Figure 3.

Figure 3

miR‐204 targets EphB2 in cultured primary hippocampal neurons. (A) miR‐204 represses the expression of luciferase‐Ephb2 3′ UTR in a dose‐dependent manner in primary hippocampal neurons. N = 3 independent cultures per treatment. Error bars indicate standard deviations. (B) The mutated 3′ UTR sequence of the luciferase‐Ephb2 3′ UTR* construct. Only the miRNA target sequences are shown. The mutated 3′ UTR contains five mismatches with miR‐204 (red). (C) Comparison of miR‐204‐induced luciferase repression in neurons transfected with wild‐type or mutant 3′ UTR sequences. SCR, Scrambled miRNA control. n = 3, error bars indicate standard deviations, *P < 0.005. (D) Effect of miR‐204 on EphB2 protein expression. Primary hippocampal neurons were transfected with miR‐204 mimic or scramble control miRNA (SCR). α‐tubulin was used as the gel loading control. Insert above: sample Western blot, n = 3. There was a significant decrease in EphB2 protein in neurons transfected with the miR‐204 mimic but not in those transfected with the scrambled control. Error bars indicate standard deviations, *P < 0.05.