Figure 5.

miR‐204 induces senescence‐like phenotype by repressing EphB2 in DIV 24 hippocampal neurons. (A) The appearance of increased SA‐ß‐Gal activity were analyzed and quantitated 21 days after transfection. Percentage of cells positive for the indicated marker is shown in histograms, which correspond to the mean. Error bars indicate standard deviations of three independent experiments from a total of at least 300 cells. *P < 0.05, n = 3 (B) Increased expression of senescent marker p16 in miR‐204‐transfected neurons. Error bars indicate standard deviations of three independent experiments from a total of at least 450 cells. *P < 0.05, n = 3.