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. 2016 Jan 13;15(2):361–369. doi: 10.1111/acel.12442

Figure 4.

Figure 4

miR‐143 negatively regulates the differentiation of mouse and human myoblasts. Expression of miR‐143 and its target gene, Igfbp5, was manipulated in undifferentiated myoblasts using miR‐143 mimic (miR‐143), anti‐miR‐143 (AM143), Igfbp5 overexpression construct (IGFBP5) and/or siRNA against Igfbp5; myotubes were stained for myosin heavy chain: MF20 ‐ green; blue – DAPI; adult – 7‐month‐old mice; old – 24‐month‐old mice; human – adult. (A) MF20 immunostaining showing myogenic differentiation regulation by miR‐143. (B) Quantification of fusion index is shown. (C) Quantification of total myotube area is shown (%). Error bars show SEM, * – P < 0.05 (compared to control), n = 3–4.