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. 2015 Dec 25;15(2):217–226. doi: 10.1111/acel.12422

Figure 2.

Figure 2

Effect of overexpression of the Drosophila melanogaster small HSP family in S2 cells on refolding heat‐inactivated firefly luciferase. Drosophila melanogaster small HSPs and firefly luciferase were coexpressed in Schneider's S2 cells. Twenty‐four hours after transfection, S2 cells were heated for 30 min at 38 °C reducing luciferase activity to < 5%. Next, cells were re‐incubated for 1 h at 25 °C to allow for (chaperone‐assisted) luciferase refolding. Luciferase activities are plotted relative to the activity in unheated cells (=100%). Data are mean ± SD of three independent experiments. Lower panel shows expression of chaperones using anti‐V5 antibodies.