Fig. 4.

The cgly serum does not detect polypeptides in the tonoplast preparation. In each panel, analysis was by SDS–PAGE and protein blot using anti-cgly serum. (A) Total proteins were extracted from leaves of wild-type (wt) or xylT–/fucT– Arabidopsis. Equal amounts of protein were analyzed. (B) Soluble (S) and microsomal (M) fractions were prepared from vacuoles or protoplasts in the presence (+) or absence (–) of Na₂CO₃. An equal proportion of each fraction, or a 10-fold dilution of each total unfractionated sample (T), was analyzed. (C) As in (B) but an equal amount of protein was analyzed for each fraction. In each panel, numbers on the left indicate the positions of molecular mass markers, in kDa.