Fig. 2.

Transcriptional activity of GhMYB108 and subcellular localization of GhMYB108–GFP fusion proteins. (A) EMSA analysis of the binding of GhMYB108 to the MBS cis-elements. GhMYB108 proteins were incubated with biotin-labeled probe (2× TAACGGAC) in the absence or presence of a 20-, 50-, or 100-fold excess of unlabeled competitor. (B) Transcriptional activation activity of GhMYB108 in Arabidopsis protoplasts. The empty vector pRT-BD and pRT-BD-VP16 were used as negative and positive control, respectively. Error bars represent the SD of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P<0.05, **P<0.01). (C) Subcellular localization of intact and truncated fusion proteins. GFP, GhMYB108–GFP, GhMYB108ΔC–GFP, and GhMYB108ΔN–GFP fusion proteins were transiently expressed in N. benthamiana leaves. GFP fluorescence was visualized by confocal microscopy. Numbers represent amino acid residues. Scale bars=20 μm. (This figure is available in colour at JXB online.)