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. 2016 Feb 11;67(6):1935–1950. doi: 10.1093/jxb/erw016

Fig. 3.

Fig. 3.

Increased susceptibility of GhMYB108-silenced cotton plants to V. dahliae. (A) Analysis of GhMYB108 expression levels. Total RNAs were extracted from leaves of cotton plants at 14 d post-agroinfiltration, and the expression level of GhMYB108 in VIGS plants was compared with that of the control plant (TRV:00). Asterisks indicate statistically significant differences, as determined by Student’s t-test (**P<0.01). (B) Disease symptoms of control (TRV:00) and GhMYB108-silenced (TRV:GhMYB108) plants infected by V. dahliae. (C) Rate of diseased plants and disease index of the control and GhMYB108-silenced plants. Error bars represent the SD of three biological replicates (n≥30). Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P<0.05). (D) Comparison of a longitudinal section of stem between control and GhMYB108-silenced cotton plants 20 d after V. dahliae infection. Arrows indicate the vascular part of the stem. (E) Fungal recovery assay. The stem sections from cotton plants 20 d after V. dahliae infection were plated on potato dextrose agar medium. Photos were taken at 6 d after plating. The number of stem sections on which the fungus grew showed the extent of fungal colonization. (This figure is available in colour at JXB online.)