Fig. 4.

Enhanced disease tolerance of Arabidopsis plants overexpressing GhMYB108. (A) Expression levels of GhMYB108 in WT (wild-type) and transgenic Arabidopsis lines (7-4, 35-3, and 39-2). (B) Symptoms of WT and GhMYB108 transgenic plants inoculated with V. dahliae for 22 d. (C and D) Rate of diseased plants and disease index of WT and transgenic plants. Error bars indicate the SD of three biological replicates with 36 plants per repeat. (E) Quantification of fungal biomass. Real-time PCR analysis was conducted to compare the transcript levels between the ITS gene (as a measure for fungal biomass) of V. dahliae and the Rubisco gene of Arabidopsis (for equilibration) at 22 d post-inoculation. Relative amounts of fungal DNA were set to 100% for the WT. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P<0.05, **P<0.01). (This figure is available in colour at JXB online.)