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. 2016 Feb 11;67(6):1935–1950. doi: 10.1093/jxb/erw016

Fig. 8.

Fig. 8.

GhMYB108 regulates the transcription of GhCML11. (A) Expression analysis of GhCML11 in control (TRV:00) and GhMYB108-silenced (TRV:GhMYB108) plants. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P<0.05). (B) EMSA of the binding of GhMYB108 to the promoter of GhCML11. The underlined sequence indicates the core motif of the MYB-binding site. (C) Analysis of the effect of GhCML11 proteins on the binding activity of GhMYB108 to the GhCML11 promoter. Anti-GST antibody against GST-tagged GhCML11 was added in the reaction to detect the presence of GhCML11 in the GhMYB108–DNA complexes. (D) Activation of GhCML11 transcription by GhMYB108. Luminescence imaging was performed 48h after co-infiltration of N. benthamiana leaves with equal amounts of Agrobacterium cells containing the indicated constructs on the left panel. (E) Quantitative analysis of luminescence intensity in (D). Error bars represent the SD (n=30) of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P<0.05). (This figure is available in colour at JXB online.)