Fig. 9.

Ca²⁺ levels in cytosol of root cells in control, GhMYB108-silenced, and GhCML11-silenced cotton plants. (A) Change in fluorescent intensity of control, GhMYB108-, and GhCML11-silenced cotton root cells treated with Fluo-4/AM at the indicated time points after inoculation with V. dahliae. Error bars represent the SD (n≥10) of three biological replicates. Asterisks indicate statistically significant differences, as determined by Student’s t-test (*P<0.05). (B) Fluorescence images of cotton root cells at 0, 4, and 60min post-inoculation with V. dahliae. The fluorescence signals were visualized by confocal microscopy. Scale bars=20 μm.