Fig. 9.

In vivo redox status of Rubisco activase in response to light in the wild type and Trx f mutant lines. The redox status of Rubisco activase from the different lines under analysis, as indicated on the left, was determined in leaf extracts from plants grown under short-day conditions and harvested at the end of the dark period (time 0), and after 1, 5, and 10min of illumination. Total leaf proteins were extracted in the presence of 10% TCA and protein thiols were alkylated with 10mM MM-PEG₂₄. Samples were fractionated in SDS-PAGE (9.5% polyacrylamide) under non-reducing conditions, transferred to nitrocellulose filters, and probed with an anti-Rubisco activase antibody; red, reduced; ox, oxidized.