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. 2016 Feb 16;67(6):2007–2022. doi: 10.1093/jxb/erw031

Fig. 8.

Fig. 8.

Functional analysis of the RHS10 downstream process. (A) Yeast two-hybrid analysis of the interaction between the extracellular (EC-D) or kinase domain (Kinase-D) of RHS10 and the whole RNS2 sequence. The EC-D includes fragments 1–235 and Kinase-D includes fragments 256–710 from the whole RHS10 sequence. ‘Empty’ is the vector control. (B) The loss-of-function RNS2 mutant grows slightly longer hairs than the wild type (WT). Root hair length was estimated with a total of 98–100 root hairs from 10 seedlings of the WT and rns2 mutant. Data represent the mean ±SE The value of rns2 is significantly (P<0.0001) different from the WT value. (C) Representative root images of WT and rns2 mutant seedlings. The scale bar is 100 μm in all images. (D) RNS2 overexpression inhibits root hair growth. Root hair length was estimated with a total of 105–717 root hairs from 11–74 seedlings from the control (Cont, ProE7:YFP) and independent RNS2 overexpression lines (RNS2ox, ProE7:RNS2). (E) Representative root images of Cont and RNS2ox transformant lines. The scale bar is 100 μm in all images. (F) Relative RNA contents from each root developmental zone of the control (Cont, ProE7:YFP), mutant (rns2 and rhs10), and transformant (E7:RHS2=ProE7:RHS2, E7:RHS10=ProE7:RHS10) seedlings. Data represent the mean ±SE from 47–49 seedlings. Differences are significant at P<0.0005 (*). Broken lines indicate fluorescence levels without SYTO dye.