Figure 2.

Co-labeling of Nestin and glial fibrillary acidic protein (GFAP) in rat spinal cord transverse sections. The Nestin positive cells were shown in red fluorescence (A), while the GFAP was stained in green fluorescence (B). All cell nuclei were counterstained in blue (C). When the images were merged, the cells co-expressing Nestin and GFAP were shown in yellow at 400× magnification (D). These study sites (the central canal and the lesion edge) were located in the yellow box area of the spinal cord transverse section at 40× magnification (E). All the Nestin positive cells at the central canal were not co-labeled with GFAP (arrows). The cells at the lesion edge co-expressed Nestin and GFAP (arrowheads).