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. 2016 Mar 9;7:265. doi: 10.3389/fmicb.2016.00265

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Copyright © 2016 Tun, Cai and Khafipour.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survivability of PEDv in two infected on-farm EMS. Using real-time RT-PCR targeted to S gene, the survivability of PEDv over time was tested based on the detectable viral RNA copies number in 1 ml of EMS at each sampling time point during fall 2014 sampling for farm-1 EMS (A), and from fall 2014 to summer 2015 sampling for farm-2 EMS (B). The bar shows average RNA copy number of PEDv in the respective layer of EMS. The error bars show the standard deviation based on 12 replicates per layer of the EMS in farm-1 and 16 replicates in farm-2. Each biological sample was analyzed in triplicate using real-time RT-PCR. ^∗∗∗P < 0.001 and ^∗∗∗∗P < 0.0001.