Table 3.

Infectivity of PEDv in the farm-1 EMS.

Sampling week/date	EMS layer	% of PEDv infective (number of PEDv infective samples/ total number of tested samples)	Infective titer (TCID50)1	Fisher’s exact test2
				X2	P-value
Week 5/23 October 2014	Top	0 (0/12)	0.0	1.06	0.58
	Middle	8.3 (1/12)	1.00E + 05
	Bottom	8.3 (1/12)	1.00E + 08
Week 7/05 November 2014	Top	0 (0/12)	0.0	8.4	0.01
	Middle	41 (5/12)	1.00E + 06 to 1.00E + 09
	Bottom	8.3 (1/12)	1.00E + 08

¹TCID₅₀: the 50% tissue culture infective dose. Greater values are indicative of greater infectivity of virus.

²Statistical comparisons were made between different layers of the EMS within each sampling time point.