Fig. 2.

Glycolysis inhibition leads to decreased ATP formation by exosomes. ATP ladder (50 to 0.4 pmole) illustrating ATPase activities of prostasomes and PC3 exosomes in absence and presence of 3.3 mM vanadate (V, ATPase inhibitor) (a). Please note the minute amounts of ATP for prostasomes in absence of vanadate (V) in sharp contrast to PC3 exosomes. Estimation of ATP formation (incubation medium always containing glucose, G), in prostasomes (1 µg protein) and PC3 exosomes (1 µg protein), in (b) absence or (c) presence of glycolytic inhibitors (IA: iodoacetate; NaF: sodium fluoride) (mean values±SD, n ≥ 3).