Fig. 3.

EVs are taken up by prostate healthy and cancer cells. Uptake of PKH67-labelled (a) prostasomes (representing 1 µg protein) and (c) PC3 exosomes (representing 1 µg protein) by prostate epithelial CRL2221 cells and PC3 cells, illustrated by confocal microscopy after 3 h of incubation (scale bar: 5 µm). Estimation of PKH67-labelled (b) prostasomes and (d) PC3 exosomes. EV uptake (measured by flow cytometry) after 1 h, 3 h and 6 h at 37°C and results are presented as percentage of positive cells and fluorescence intensity (mean values±SEM, n=3)