Fig. 4.

Estimation of prostasome and exosome uptake by recipient cells being dependent on V-ATPase. (a) CRL2221 cells and (b) PC3 cells were incubated with PKH67-labelled prostasomes (representing 1 µg protein) or PC3 exosomes (representing 1 µg protein) in absence or presence of bafilomycin (BafA1, 10 nM), 2-deoxyglucose (2-DG, 10 mM) and/or oleic acid (OA) (100 µM) for 3 h at 37°C. Fluorescence intensity was analysed by flow cytometry. Results are expressed as a fold relationship to the control (exosomes without treatment) (mean values±SEM, n=3).