Table 1.

Arginine Biosynthesis metabolites identified from planktonic and biofilm data sets

Peak number	Metabolite	Elemental formula	KEGG IDa	Metabolite codeb	Mass [M–H] (Da)	Rct (sec)	Log2 fold changed
2782	Aspartate	C4H7NO4	C00049	${\text{HRMS}}_{\text{PL}}^1$	132.0302	749.1	1.577
2873	Glutamate	C5H9NO4	C00025	${\text{HRMS}}_{\text{a}}^1$ Rta	146.0458	725.7	1.0159
2981	Glutamine	C5H10N2O3	C00064	${\text{HRMS}}_{\text{a}}^1$ Rta	145.0618	781.3	n/a(e)
3171	Citrulline	C6H13N3O3	C00327	${\text{HRMS}}_{\text{a}}^1$ Rta	174.0884	813.1	3.5413
3252	N-Acetyl-l-glutamate	C7H11NO5	C00624	${\text{HRMS}}_{\text{a}}^1$ Rta	188.0564	691.1	4.3654
3505	l-Arginosuccinate	C10H18N4O6	C03406	${\text{HRMS}}_{\text{PL}}^1$	289.1156	800.5	n/af
3608	N-Acetyl-l-citrulline	C8H15N3O4	C15532	${\text{HRMS}}_{\text{PL}}^1$	216.099	594.1	5.049
3636	N-Acetyl-ornithine	C7H14N2O3	C00437	${\text{HRMS}}_{\text{a}}^1$ Rta	173.0931	791.2	1.068
3838	Arginine	C6H14N4O2	C00062	${\text{HRMS}}_{\text{PL}}^1$	173.1044	1304.1	1.3781

^aSee reference Kanehisa and Goto 2000

^bSee reference Sumner et al. 2014

^cRetention time

^dLog₂ fold change in expression of metabolite between planktonic and biofilm data sets

^eNot available, fold change unable to be formulated as peak intensities did not significantly change between sample sets

^fNot available, fold change unable to be formulated as only detected in the planktonic data set and not in the biofilm data set