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. 2016 Feb 23;26(3):275–287. doi: 10.1038/cr.2016.20

Figure 3.

Figure 3

Embryogenesis defects in YAP-deleted embryos. (A) Morphology of zygotes derived from WT and Yap1fl/fl;Gdf9-Cre females after hCG injection and successful mating. Scale bar, 50 μm. (B) Pronucleus formation rates of WT and Yap1♀−/♂+ zygotes. Error bar indicates SEM. (C, D) Immunofluorescence showing RNA polymerase II phosphorylation (pS2) (C) and histone H3 trimethylation at lysine 4 (H3K4me3) (D) in WT and Yap1♀−/♂+ embryos at the two-cell stage. Scale bar, 10 μm. (E) Immunofluorescence of Nanog and CDX2 in WT and Yap1♀−/♂+ embryos at the time when WT embryos develop to the blastocyst stage. TE, trophectoderm; ICM, inner cell mass. Scale bar, 10 μm. (F) Immunofluorescence of OCT4 in WT and Yap1♀−/♂+ embryos at the time when WT embryos are at the blastocyst stage. Scale bar, 10 μm.