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. 2016 Mar 9;36(10):2915–2925. doi: 10.1523/JNEUROSCI.3833-15.2016

Figure 4.

Figure 4.

GPR179 and R9AP physically interact in vivo and in transfected cells. A, Immunoprecipitation of R9AP from retinas following detection of coeluting proteins by Western blotting. R9AP KO retinas were used as a negative control in a parallel experiment. GPR179 and RGS11 coimmunoprecipitate with R9AP from WT, but not from the R9AP KO retinas. B, Analysis of R9AP and GPR179 coimmunoprecipitation in transfected HEK293 cells. Top, Antibodies against R9AP are able to specifically coimmunoprecipitate GPR179. Bottom, Antibodies against GPR179 are able to coimmunoprecipitate R9AP.