Table 2.

Concentration–response analysis reveals that chronic menthol alters α4β2 nAChR stoichiometry

	Treatment condition
	α4-GFPβ2 no drug	α4-GFPβ2 + menthol
EC50 value (higher sensitivity)	1.8 ± 3.1 μm	7.8 ± 4.9 μm
nh (higher)	0.645	0.57
EC50 value (lower sensitivity)	41.9 ± 4.7 μma	95.2 ± 14.0 μma,b
nh (lower)	2.2	2.2
Higher fraction	0.49	0.14

Values are represented as mean ± SEM.

^aEC₅₀ values are statistically different from HS α4-GFPβ2 nAChRs (no drug). p < 0.005, unpaired t test.

^bEC₅₀ values are statistically different from lower-sensitivity α4-GFPβ2 nAChRs (no drug). p < 0.005, unpaired t test.