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. 2016 Mar 9;36(10):3079–3091. doi: 10.1523/JNEUROSCI.4012-15.2016

Figure 2.

Figure 2.

Loss of Cdk5 activity blocks the ability of dbcAMP and putrescine to overcome inhibition by myelin-associated inhibitors. A, Quantification of neurite outgrowth for CGNs and DRG neurons primed with 100 μm putrescine and 10 μm roscovitine and plated on monolayers of control or MAG-expressing CHO cells. B, Representative images and quantification of neurite outgrowth for DRG neurons primed with 100 μm putrescine and 10 μm roscovitine and plated on CNS myelin. C, Western blot of DRG neurons transduced with HSV expressing LacZ, p35, or dominant negative Cdk5 (DNCdk5). D, Quantification of neurite outgrowth for DRG neurons transduced with HSV expressing LacZ, p35, and DNCdk5, treated with 1 mm dbcAMP and plated on monolayers of control or MAG-expressing CHO cells. E, Quantification of neurite outgrowth for DRG neurons transduced with HSV expressing LacZ and DNCdk5, treated with 100 μm putrescine and plated on monolayers of control or MAG-expressing CHO cells. For all experiments, neurite outgrowth was measured from a minimum of 200 neurons for each treatment. Graphs represent the average length of the longest neurite per neuron (depicted as percentage of control) ± SEM. *p < 0.05; ***p < 0.001.