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. 2016 Mar 9;36(10):3079–3091. doi: 10.1523/JNEUROSCI.4012-15.2016

Figure 5.

Figure 5.

Putrescine-mediated induction of p35 expression and reversal of MAG-mediated inhibition are translation dependent. A, Western blots of CGNs treated with 200 ng/ml BDNF, 100 μm putrescine, and 10 μm U0126 for the times indicated. B, Western blots of CGNs treated with 100 μm putrescine and 5 μm DRB for 20 h. C, Western blots of CGNs treated with 100 μm putrescine and 0.5 μg/ml cycloheximide for 20 h. D, Quantification of neurite outgrowth for CGNs primed with 1 mm dbcAMP, 100 μm putrescine, 5 μm DRB, and 0.5 μg/ml cycloheximide and plated on monolayers of control or MAG-expressing CHO cells. Neurite outgrowth was measured from a minimum of 200 neurons for each treatment. The graph represents the average length of the longest neurite per neuron (depicted as percentage of control) ± SEM. ***p < 0.001. ns, Not significant.